WebJun 11, 2024 · The engineered Xa23p1.0 was, subsequently, cloned into the transformation-competent artificial chromosome vector pYLTAC380H [ 38] and introduced into rice variety Nipponbare (Nip) through an Agrobacterium-mediated transformation. WebJun 29, 2024 · Functional genomics, synthetic biology and metabolic engineering require efficient tools to deliver long DNA fragments or multiple gene constructs. Although numerous DNA assembly methods exist, most ...
Callus specific promoter Pcsp and application thereof
http://www.ahs8.com/2024/0712/c11310a320903/page.htm WebSep 2, 2014 · POD activity was measured according to Maehly ( 1954) based on an increase in absorbance at 470 nm. The assay solution contained 50 mM phosphate buffer (pH 7.0), 0.3 % H 2 O 2, 0.2 % guaiacol, and 50 μL of enzyme extract. One unit of peroxidase activity equaled a change of 0.01 absorbance unit/min. Determination of proline content hdfs core-site not found
Ectopic Expression of Executor Gene Xa23 Enhances Resistance …
WebAug 1, 2013 · The products were cloned into pYL322-d1-TobRB7, pYL322-d1-CaMV35S, or pYL322-d1-LMX5 and then recombined into pYLTAC380H, which contained the HPTII gene as a selectable marker with the Cre/loxP system. The constructs were transformed into Agrobacterium strain LBA4404 via the freeze/thaw method (Holsters et al., 1978). Web(c) Not I-digestion analysis of pYLTAC380H-CDGAeG, pYLTAC380H-HPs/CDGAeG and pYLTAC380H-GSs/HPs/ CDGAeG constructs in E. coli and A. tumefaciens. M: DNA … WebNew England Biolabs nb bsrdi Nb Bsrdi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. hdfs-config.sh: no such file or directory